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Saturday | May 21 | 1:00 p.m. ET | 
Fetzer Field | Chapel Hill, N.C. | First Round 
#6 Notre Dame Fighting Irish14-6 (4-3, T-3rd ACC) – At-Large
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- Coach: Christine Halfpenny (5 NCAAs)
- Ranks (IWLCA / Inside Lacrosse / RPI): 6 / 7 / 7
- Key Wins: UVA, Duke, Northwestern, Stanford, BC, Princeton, Louisville
- NCAA Record: 7-11 (11th appearance)
- Last Appearance: NCAA Second Round (2015)
- Best Finish: NCAA Semifinals (2006)
#3 North Carolina Tar Heels17-2 (7-0, 1st ACC) – ACC Champion
- Coach: Jenny Levy (8 Final Fours, 1 title)
- Ranks (IWLCA / Inside Lacrosse / RPI): 3 / 3 / 2
- Key Wins: UVA, Northwestern, Duke, Notre Dame, Syracuse
- NCAA Record: 25-16 (18th appearance)
- Last Appearance: NCAA Runner-Up (2015)
- Best Finish: NCAA Champion (2013)